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Stencils can generate opacity gradients in thin alginate gels. (A) Two–sided adhesive silicone sheets cut with patterns were doubly stacked creating a 2 mm thick reservoir to hold sparsely cross-linked alginate gels (20 mg ml −1 alginate gelled with 20 mM CaSO 4 ). This reservoir was adhered to a <t>polystyrene</t> cell culture dish. (A) stencil was adhered to the top silicone sheet and the cell culture dish was filled with 80 mM CaCl 2 to crosslink alginate gel exposed to the solution. (B) Image of two chambers attached to one 35 mm polystyrene cell culture dish. The silicone well is white. (C) Images were taken before crosslinking (0 h) and after crosslinking (8 h) with 80 mM CaCl 2 .
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Stencils can generate opacity gradients in thin alginate gels. (A) Two–sided adhesive silicone sheets cut with patterns were doubly stacked creating a 2 mm thick reservoir to hold sparsely cross-linked alginate gels (20 mg ml −1 alginate gelled with 20 mM CaSO 4 ). This reservoir was adhered to a <t>polystyrene</t> cell culture dish. (A) stencil was adhered to the top silicone sheet and the cell culture dish was filled with 80 mM CaCl 2 to crosslink alginate gel exposed to the solution. (B) Image of two chambers attached to one 35 mm polystyrene cell culture dish. The silicone well is white. (C) Images were taken before crosslinking (0 h) and after crosslinking (8 h) with 80 mM CaCl 2 .
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Stencils can generate opacity gradients in thin alginate gels. (A) Two–sided adhesive silicone sheets cut with patterns were doubly stacked creating a 2 mm thick reservoir to hold sparsely cross-linked alginate gels (20 mg ml −1 alginate gelled with 20 mM CaSO 4 ). This reservoir was adhered to a <t>polystyrene</t> cell culture dish. (A) stencil was adhered to the top silicone sheet and the cell culture dish was filled with 80 mM CaCl 2 to crosslink alginate gel exposed to the solution. (B) Image of two chambers attached to one 35 mm polystyrene cell culture dish. The silicone well is white. (C) Images were taken before crosslinking (0 h) and after crosslinking (8 h) with 80 mM CaCl 2 .
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Stencils can generate opacity gradients in thin alginate gels. (A) Two–sided adhesive silicone sheets cut with patterns were doubly stacked creating a 2 mm thick reservoir to hold sparsely cross-linked alginate gels (20 mg ml −1 alginate gelled with 20 mM CaSO 4 ). This reservoir was adhered to a <t>polystyrene</t> cell culture dish. (A) stencil was adhered to the top silicone sheet and the cell culture dish was filled with 80 mM CaCl 2 to crosslink alginate gel exposed to the solution. (B) Image of two chambers attached to one 35 mm polystyrene cell culture dish. The silicone well is white. (C) Images were taken before crosslinking (0 h) and after crosslinking (8 h) with 80 mM CaCl 2 .
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standard monodisperse polystyrene shodex standard sm  (Shodex)
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Stencils can generate opacity gradients in thin alginate gels. (A) Two–sided adhesive silicone sheets cut with patterns were doubly stacked creating a 2 mm thick reservoir to hold sparsely cross-linked alginate gels (20 mg ml −1 alginate gelled with 20 mM CaSO 4 ). This reservoir was adhered to a <t>polystyrene</t> cell culture dish. (A) stencil was adhered to the top silicone sheet and the cell culture dish was filled with 80 mM CaCl 2 to crosslink alginate gel exposed to the solution. (B) Image of two chambers attached to one 35 mm polystyrene cell culture dish. The silicone well is white. (C) Images were taken before crosslinking (0 h) and after crosslinking (8 h) with 80 mM CaCl 2 .
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Stencils can generate opacity gradients in thin alginate gels. (A) Two–sided adhesive silicone sheets cut with patterns were doubly stacked creating a 2 mm thick reservoir to hold sparsely cross-linked alginate gels (20 mg ml −1 alginate gelled with 20 mM CaSO 4 ). This reservoir was adhered to a polystyrene cell culture dish. (A) stencil was adhered to the top silicone sheet and the cell culture dish was filled with 80 mM CaCl 2 to crosslink alginate gel exposed to the solution. (B) Image of two chambers attached to one 35 mm polystyrene cell culture dish. The silicone well is white. (C) Images were taken before crosslinking (0 h) and after crosslinking (8 h) with 80 mM CaCl 2 .

Journal: Biomedical Materials (Bristol, England)

Article Title: Leveraging crosslinker diffusion to template stiffness gradients in alginate hydrogels

doi: 10.1088/1748-605X/ae1ab7

Figure Lengend Snippet: Stencils can generate opacity gradients in thin alginate gels. (A) Two–sided adhesive silicone sheets cut with patterns were doubly stacked creating a 2 mm thick reservoir to hold sparsely cross-linked alginate gels (20 mg ml −1 alginate gelled with 20 mM CaSO 4 ). This reservoir was adhered to a polystyrene cell culture dish. (A) stencil was adhered to the top silicone sheet and the cell culture dish was filled with 80 mM CaCl 2 to crosslink alginate gel exposed to the solution. (B) Image of two chambers attached to one 35 mm polystyrene cell culture dish. The silicone well is white. (C) Images were taken before crosslinking (0 h) and after crosslinking (8 h) with 80 mM CaCl 2 .

Article Snippet: The kinetics and extent of crosslinking alginate with CaSO 4 and CaCl 2 were analyzed using a UV-VIS Spectrophotometer (Varian Cary 50 Bio) and polystyrene disposable cuvettes (Semi-Micro, 1 cm path, 1.5 ml, 12.5 mm square × 45 mm high; Bio-Rad, 2239 955).

Techniques: Adhesive, Cell Culture